normal human colon epithelial cell model  (ATCC)

Journal: Journal of Biological Engineering

Article Title: Hydrogel-mediated tri-modal nanoplatform for localized colorectal cancer therapy via smart chemo–photothermal–radiotherapy

doi: 10.1186/s13036-026-00633-0

Figure Lengend Snippet: Cytotoxic response and intracellular ROS levels induced by the multimodal hydrogel nanoplatform in CRC and normal colon cell models. (A) Dose–response curve of free 5-FU in HCT-116 cells after 48 h, determined by MTT assay and used to define the experimental IC₅₀. Cell viability was normalized to the Ctrl. For visualization on a logarithmic x-axis, the Ctrl condition is plotted at a nominal concentration of 0.1 µg mL⁻¹. (B–D) MTT-based cell viability after 48 h treatment in (B) HCT-116, (C) SW480, and (D) normal human colon epithelial NCM460 cells. Treatment groups include Ctrl, free 5-FU (5FU), non-targeted nanoparticles (NP), HA-targeted nanoparticles (tNP), blank hydrogel (Gel), hydrogel-loaded targeted nanoparticles (Gel-tNP), and Gel-tNP combined with near-infrared irradiation (NIR, 808 nm), X-ray irradiation (XR, 2 Gy), or both. All treatments were administered at an equivalent 5-FU concentration corresponding to the IC₅₀ determined in panel (A). (E) Intracellular ROS levels in HCT-116 cells measured after 48 h using CellROX™ Deep Red and expressed as fold change relative to Ctrl. Data are mean ± SD ( n = 3). Statistical significance was assessed by one-way ANOVA with multiple-comparisons post-hoc testing. Asterisks indicate comparisons vs. Ctrl (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Brackets indicate the specific intergroup comparisons shown (e.g., dual-modal vs. tri-modal where indicated). “ns” denotes not significant

Article Snippet: The cytotoxicity of all treatment groups was evaluated using an MTT assay in two CRC cell lines and one normal human colon epithelial cell model. HCT-116 human colorectal carcinoma cells (ATCC ® CCL-247TM, American Type Culture Collection, USA) and SW480 human colorectal adenocarcinoma cells (ATCC ® CCL-228TM, American Type Culture Collection, USA) were used as CRC models, and normal human colon epithelial cells NCM460 (NCM460DTM; INCELL Corporation LLC, San Antonio, TX, USA) were used to provide an initial in-vitro assessment of relative tolerance in non-malignant colon epithelium.

Techniques: MTT Assay, Concentration Assay, Irradiation

Journal: Molecular Therapy Oncology

Article Title: Tackling cancer heterogeneity with systemically delivered oncolytic adenoviruses transcriptionally targeted with hybrid promoters

doi: 10.1016/j.omton.2025.201073

Figure Lengend Snippet: mRNA and protein expression levels of selected candidates in human CRC cell lines and patients’ samples (A–F) mRNA expression levels of the six selected candidates for inclusion in the hybrid TSP. Quantitative PCR (qPCR) analysis was performed using specific primers for each candidate gene (vWA2, A33, FSCN1, NQO1, PDL1, and AQP5) in four human colorectal cancer cell lines (T84, HCT116, HT29, and LoVo) and in normal human colonic epithelial cells (CCD841). qPCR data are presented as relative quantification (RQ = 2 −ΔΔCt ). Statistical analysis was performed using one-way ANOVA. Data represent the mean of three independent experiments. (G) Immunohistochemical analysis of gpA33 and vWA2 expression was performed on paraffin-embedded primary and metastatic CRC tissue samples. (a and b) Representative images of primary tumor sections stained with an anti-gpA33 antibody and developed as described in the . (c and d) Representative images of primary tumor sections stained with an anti-vWA2 antibody under the same conditions. (e–g) Representative images of uterine, cutaneous, and liver metastases, respectively, stained with the anti-gpA33 antibody. (h–j) Corresponding metastatic tissue sections (uterus, skin, and liver, respectively) stained with an anti-vWA2 antibody. All images were acquired at an original magnification of 200×. Scale bar: 50 μm.

Article Snippet: Human CRC cell lines (LoVo, T84, HCT116, and HT29), normal colon epithelial cells (CCD841), human embryonic kidney cells (HEK293), human fetal lung fibroblasts (WI-38), and human microendothelial cells (HMEC-1) were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative Proteomics, Immunohistochemical staining, Staining

Journal: Molecular Therapy Oncology

Article Title: Tackling cancer heterogeneity with systemically delivered oncolytic adenoviruses transcriptionally targeted with hybrid promoters

doi: 10.1016/j.omton.2025.201073

Figure Lengend Snippet: AR2015 lytic activity in vitro compared to single TSP-driven OAds (A–I) In vitro lytic activity of the OAds AR2015, AV22EL, and AV636, as well as Ad5WT (positive control), in CRC cell lines LoVo, T84, HT29, and HCT116, normal human colonic epithelial cells (CCD841), human fetal lung fibroblasts (WI-38), human microendothelial cells (HMEC-1), and human melanoma cells (A375 and SB2). Cells (1 × 10 4 ) were seeded in 24-well plates and infected 24 h later with increasing multiplicities of infection (MOIs: 0–100). After 6 days, cell viability was assessed using the MTS assay and expressed as mean ± SD ( n = 3), with the viability of uninfected control cells set to 100%. Two-way ANOVA was performed for statistical analysis, followed by Dunnett’s test (vs. Ad(F5)WT). (J) Comparative replication kinetics of AR2015 and AV22EL in CRC cell lines (LoVo, T84, HT29, and HCT116) and normal CCD841 cells. Cells were infected at an MOI of 100, and samples were collected at 5 h (baseline) and 72 h post-infection. Viral replication was quantified by qPCR targeting the adenoviral E4 gene. Data are presented as a fold increase in E4 levels (72 vs. 5 h). (K) Expression of adenoviral E1A protein in LoVo cells at 8 and 24 h post-infection with AV22EL, AR2015, or Ad5WT. Protein levels were assessed by western blot, with β-actin used as a loading control.

Article Snippet: Human CRC cell lines (LoVo, T84, HCT116, and HT29), normal colon epithelial cells (CCD841), human embryonic kidney cells (HEK293), human fetal lung fibroblasts (WI-38), and human microendothelial cells (HMEC-1) were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Activity Assay, In Vitro, Positive Control, Infection, MTS Assay, Control, Expressing, Western Blot